Summary: hTERT-RPE1 cells are genetically stable near diploid Fixed Blade Tactical cells widely used to model cell division, DNA repair, or ciliogenesis in a non-transformed context.However, poor transfectability and limited homology-directed repair capacity hamper their amenability to gene editing.Here, we describe a protocol for rapid and efficient generation of diverse homozygous knockins.In contrast AIR RIFLE to other approaches, this strategy bypasses the need for molecular cloning.
Our approach can also be applied to a variety of cell types including cancer and induced pluripotent stem cells (iPSCs).